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human aortic endothelial cells haec  (ATCC)


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    Structured Review

    ATCC human aortic endothelial cells haec
    Human Aortic Endothelial Cells Haec, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 445 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+aortic+endothelial+cells+haecs/Primary+Aortic+Endothelial+Cells%3B+Normal%2C+Human/pm42127698-89-9-14
    Average 99 stars, based on 445 article reviews
    human aortic endothelial cells haec - by Bioz Stars, 2026-09
    99/100 stars

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    Cell Culture:

    Article Title: M2 macrophage-derived extracellular vesicles protect against abdominal aortic aneurysm by modulating macrophage polarization through miR221-5p
    Article Snippet: .. To polarize M1 macrophages, BMDMs were stimulated with lipopolysaccharide (LPS) (10 ng/ml, Sigma-Aldrich, MO, USA) and interferon-γ (IFN-γ, 10 ng/ml, Sigma-Aldrich) for 24 h. M2 polarization was induced by IL-4 (20 ng/ml; PeproTech, NJ, USA) for 24 h. Human aortic endothelial cells (HAECs) and THP-1 cells (PCS-100–011, TIB-202, ATCC, USA) were cultured in endothelial cell medium (ECM) or Dulbecco’s modified Eagle medium (DMEM). .. BMDMs were treated with M2-EVs or M2-miR221-5p-ASOs prior to stimulation with LPS (1 μg/ml) for 24 h. miR221-5p, ASOs of miR221-5p (miR221-5p-ASOs), or PP-1α plasmid was transfected to BMDMs using LipofectamineTM 3000 transfection reagent (Invitrogen, CA, USA) 24 h before stimulation.

    Article Title: A Patient-Specific 3D Printed Carotid Artery Model Integrating Vascular Structure, Flow and Endothelium Responses
    Article Snippet: The media flow was driven by a peristaltic pump (IP65 ISMATEC, Masterflex, Gelsenkirchen, Germany). .. Human aortic endothelial cells (hAECs) (TeloHAEC, CRL-4052TM, ATCC, USA) and human aortic smooth muscle cells (hASMCs) (AoSMC, CC-2571, Lonza, Switzerland) were routinely maintained in T175 cell culture flasks at 37.5°C and 5% CO2. hAECs were grown in Vascular Cell Basal Medium supplemented with the Endothelial Cell Growth Kit-VEGF (PCS-100-041, ATCC, USA) and hASMCs were grown in the Smooth Muscle Cell Growth Medium 2-Bullet Kit (CC-3182, Lonza, Switzerland). .. 1% penicillin-streptomycin (ThermoFisher, Australia) was supplemented in all media.

    Article Title: Ginsenoside Rb1 mitigates atherosclerosis in part through modulating FTO-mediated m 6 A RNA modification in NETs-induced endothelial activation
    Article Snippet: Isolated neutrophils were cultured in 10-cm cell culture dishes at 5 × 10 5 cells/mL, followed by exposure to PMA (100 nM) for 4 h. Afterward, the cell culture dishes were thoroughly washed and NETs were collected by vigorous agitation, followed by 5-min centrifugation at 300 × g. The supernatants were collected and subjected to 20-min centrifugation at 12,000 × g at 4 C. After resuspension, the DNA content was estimated using a BioSpec-nano Spectrophotometer (Shimadzu, Japan). .. Human aortic endothelial cells (HAECs) (Shanghai Institute of Quicell Biotechnology, China) were cultured in ECM supplemented with reagents from the endothelial cell growth kit (ATCC, United States). ..

    Article Title: Transsulfuration metabolism is essential for ferroptosis resistance in quiescent endothelial cells.
    Article Snippet: HUVECs were cultured until the sixth passage in 0.2% pre-coated gelatin (Sigma, cat. N°G2500) plates. .. Human aortic endothelial cells (HAECs) were purchased form American Type Culture Collection (ATCC, cat. N° CRL-4052) and cultured in Endothelial Cell Growth Medium 2 (PromoCell) supplemented with 100 U/ml penicillin and 100μg/mL streptomycin. .. MS1 were purchased from ATCC (cat. N° CRL-2279) and maintained in DMEM 10 % FBS supplemented with 100 U/ml penicillin and 100μg/mL streptomycin.

    Modification:

    Article Title: M2 macrophage-derived extracellular vesicles protect against abdominal aortic aneurysm by modulating macrophage polarization through miR221-5p
    Article Snippet: .. To polarize M1 macrophages, BMDMs were stimulated with lipopolysaccharide (LPS) (10 ng/ml, Sigma-Aldrich, MO, USA) and interferon-γ (IFN-γ, 10 ng/ml, Sigma-Aldrich) for 24 h. M2 polarization was induced by IL-4 (20 ng/ml; PeproTech, NJ, USA) for 24 h. Human aortic endothelial cells (HAECs) and THP-1 cells (PCS-100–011, TIB-202, ATCC, USA) were cultured in endothelial cell medium (ECM) or Dulbecco’s modified Eagle medium (DMEM). .. BMDMs were treated with M2-EVs or M2-miR221-5p-ASOs prior to stimulation with LPS (1 μg/ml) for 24 h. miR221-5p, ASOs of miR221-5p (miR221-5p-ASOs), or PP-1α plasmid was transfected to BMDMs using LipofectamineTM 3000 transfection reagent (Invitrogen, CA, USA) 24 h before stimulation.



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